| Gene Name | Rab4b |
| HF Protein Name | Ras-related protein Rab-4B |
| HF Function | |
| Uniprot ID | P61018 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 53916 |
| Host Factor (HF) Name in Paper | Rab4b |
| Gene synonyms | RAB4 |
| Ensemble Gene ID | ENSG00000167578 |
| Ensemble Transcript | ENST00000357052.8 [P61018-1];ENST00000594800.5 [P61018-1] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0003924, GO:0003925, GO:0005525, GO:0005829, GO:0005886, GO:0015031, GO:0016192, GO:0030100, GO:0030667, GO:0031901, GO:0032482, GO:0032593, GO:0034058, GO:0034498, GO:0046323, GO:0048471, GO:0055037, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 612945 |
| PANTHER ID | PTHR47979 |
| PDB ID(s) | 2O52, |
| pfam ID | PF00071, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Japanese encephalitis virus |
| Virus Short Name | JEV |
| Order | Amarillovirales |
| Virus Family | Flaviviridae |
| Virus Subfamily | N.A. |
| Genus | Flavivirus |
| Species | Japanese encephalitis virus |
| Host | Vertebrates |
| Cell Tropism | Neurons |
| Associated Disease | Encephalitis |
| Mode of Transmission | Sexual contact, blood, breast feeding |
| VIPR DB link | https://www.viprbrc.org/brc/vipr_search.do?species=Japanese_encephalitis_virus |
| ICTV DB link | https://ictv.global/report/183/flaviviridae |
| Virus Host DB link |
| Paper Title | Host Factor Rab4b Promotes Japanese Encephalitis Virus Replication |
| Author's Name | Qin Zhao, Chang Miao, Yi-Ting Chen, Long-Yue Zhu, Ya-Ting Zhang, Sai-Qi Luo, Yu-Luo Wang, Zhu-Ming Zhu, Xinfeng Han, Yiping Wen, Rui Wu, Senyan Du, Qi-Gui Yan, Xiaobo Huang, Shan Zhao, Yi-Fei Lang, Yiping Wang, Yi Zheng, Fei Zhao, San-Jie Cao |
| Journal Name | Microorganisms |
| Pubmed ID | 39338478 |
| Abstract | Although the Japanese encephalitis virus (JEV) infects various cell types, its receptor molecules are still not clearly understood. In our laboratory's prior research, Rab4b was identified as a potential host factor that facilitates JEV infection in PK15 cells, utilizing a genome-wide CRISPR/Cas9 knockout library (PK-15-GeCKO). To further explore the effect of Rab4b on JEV replication, we used the Rab4b knockout PK15 cell line using the CRISPR/Cas9 technology and overexpressing the Rab4b PK15 cell line, with IFA, RT-qPCR, and Western blot to study the effect of Rab4b on viral replication in the whole life cycle of the JEV. The results show that the knockout of Rab4b inhibited the replication of the JEV in PK15 cells, and the overexpression of Rab4b promoted the replication of the JEV in PK15 cell lines. Furthermore, we demonstrated for the first time that host factor Rab4b facilitates the adsorption, internalization, assembly, and release of the JEV, thereby promoting JEV replication. This study enriches the regulatory network between the JEV and host factors and lays the experimental foundation for further understanding of the function of the Rab4b protein. |
| Used Model | PK15, PK15-Rab4b-K.O.,PK15-Rab4b-O.E. |
| DOI | 10.3390/microorganisms12091804 |
