| Gene Name | SUN2 |
| HF Protein Name | Sad1 and UNC84 domain-containing protein 2 |
| HF Function | |
| Uniprot ID | Q9UH99 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 25777 |
| Host Factor (HF) Name in Paper | SUN2 |
| Gene synonyms | FRIGG KIAA0668 RAB5IP UNC84B |
| Ensemble Gene ID | ENSG00000100242 |
| Ensemble Transcript | ENST00000405018.5 [Q9UH99-2];ENST00000405510.5 [Q9UH99-1];ENST00000406622.5 [Q9UH99-1];ENST00000689035.1 [Q9UH99-1] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000781, GO:0000794, GO:0005521, GO:0005635, GO:0005637, GO:0007052, GO:0007097, GO:0008017, GO:0010008, GO:0021817, GO:0030335, GO:0031022, GO:0031965, GO:0034993, GO:0042802, GO:0043495, GO:0051321, GO:0051642, GO:0090292, GO:0140444, |
| MINT ID | Q9UH99 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 613569 |
| PANTHER ID | PTHR12911;PTHR12911:SF22 |
| PDB ID(s) | 3UNP, 4DXR, 4DXS, 4DXT, 4FI9, 6WMD, 6WME, 6WMF, 6WMG, |
| pfam ID | PF18580, PF07738, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Japanese encephalitis virus |
| Virus Short Name | JEV |
| Order | Amarillovirales |
| Virus Family | Flaviviridae |
| Virus Subfamily | N.A. |
| Genus | Flavivirus |
| Species | Japanese encephalitis virus |
| Host | Vertebrates |
| Cell Tropism | Neurons |
| Associated Disease | Encephalitis |
| Mode of Transmission | Sexual contact, blood, breast feeding |
| VIPR DB link | https://www.viprbrc.org/brc/vipr_search.do?species=Japanese_encephalitis_virus |
| ICTV DB link | https://ictv.global/report/183/flaviviridae |
| Virus Host DB link |
| Paper Title | Nuclear membrane protein SUN2 promotes replication of flaviviruses through modulating cytoskeleton reorganization mediated by NS1 |
| Author's Name | Yanxia Huang # 1 2 3, Qinyu Peng # 1 2, Xu Tian # 1 2, Cancan Chen 4, Xuanfeng Zhu 1 2, Changbai Huang 1 2, Zhiting Huo 1 2, Yang Liu 5, Chao Yang 6 7, Chao Liu 8 9, Ping Zhang 10 11 |
| Journal Name | Nat Commun |
| Pubmed ID | 38177122 |
| Abstract | Cytoskeleton is extensively recruited by flaviviruses for their infection. In this study, we uncovered an essential role of a nuclear membrane protein, SAD1/UNC84 domain protein 2 (SUN2) linking cytoskeleton and nucleoskeleton in the flavivirus replication. CRISPR/Cas9-mediated knockout of SUN2, but not SUN1, significantly reduces the replication of Zika virus (ZIKV), dengue virus (DENV), and Japanese encephalitis virus (JEV). In contrast, SUN2 does not affect the infection of non-flaviviridae RNA viruses. All three regions of SUN2 are required for its proviral effect. Mechanistically, SUN2 facilitates rearrangement of cytoskeleton and formation of replication organelles induced by viral infection, and hence promotes viral RNA synthesis. SUN2 is required for the interaction between cytoskeleton actin and ZIKV nonstructural protein 1 (NS1). Expression of dominant negative Nesprin-1 and Nesprin-2, which connect SUN2 to cytoskeleton proteins, alleviates the interaction between actin and NS1 and reduces viral replication levels. In a neonatal mouse infection model, SUN2 knockout dramatically alleviates the in vivo ZIKV replication and development of neuropathology. This work elucidates that recruitment of cytoskeleton proteins by flavivirus is coordinated by nuclear membrane proteins SUN2 and Nesprins, providing evidence for a link between nuclear membrane proteins and flavivirus infection. |
| Used Model | Huh7, A549, HeLa, 293T, Vero, BHK-21, C6/36 |
| DOI | 10.1038/s41467-023-44580-6 |
