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Virus Details


VHFID10096

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name C19orf66
HF Protein Name Interferon-Stimulated Gene 66 (ISG66) or Shiftless
HF Function
Uniprot ID Q9BXM7
Protein Sequence View Fasta Sequence
NCBI Gene ID 65018
Host Factor (HF) Name in Paper C19orf66
Gene synonyms N.A.
Ensemble Gene ID ENSG00000158828
Ensemble Transcript ENST00000321556.5 [Q9BXM7-1]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0000287, GO:0000422, GO:0000423, GO:0000785, GO:0002020, GO:0002082, GO:0002931, GO:0004672, GO:0004674, GO:0004676, GO:0004677, GO:0004679, GO:0004694, GO:0004711, GO:0005524, GO:0005634, GO:0005737, GO:0005739, GO:0005741, GO:0005743, GO:0005758, GO:0005783, GO:0005829, GO:0005856, GO:0006468, GO:0006511, GO:0006979, GO:0007005, GO:0010310, GO:0010629, GO:0010821, GO:0014046, GO:0016020, GO:0016236, GO:0016239, GO:0016242, GO:0016301, GO:0016504, GO:0016567, GO:0022904, GO:0030097, GO:0030424, GO:0030426, GO:0031396, GO:0031398, GO:0031625, GO:0032148, GO:0032226, GO:0033138, GO:0033603, GO:0034599, GO:0035175, GO:0035402, GO:0035403, GO:0035556, GO:0035979, GO:0036289, GO:0038203, GO:0042981, GO:0043123, GO:0043254, GO:0043422, GO:0043524, GO:0044022, GO:0044023, GO:0044024, GO:0044025, GO:0044297, GO:0044877, GO:0045727, GO:0045944, GO:0046329, GO:0048471, GO:0050821, GO:0051443, GO:0051881, GO:0051897, GO:0055131, GO:0061136, GO:0071456, GO:0072354, GO:0072371, GO:0072518, GO:0072655, GO:0072656, GO:0090141, GO:0090200, GO:0090258, GO:0097237, GO:0097413, GO:0097449, GO:0099074, GO:0106310, GO:0140823, GO:0140855, GO:0140857, GO:1900407, GO:1901525, GO:1902803, GO:1902902, GO:1902958, GO:1903146, GO:1903214, GO:1903298, GO:1903377, GO:1903384, GO:1903751, GO:1903852, GO:1903955, GO:1904544, GO:1904841, GO:1904881, GO:1905091, GO:1990244, GO:2000377, GO:2000378, GO:2001171, GO:2001243,
MINT ID Q9BXM7
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 168600;605909;608309
PANTHER ID PTHR22972;PTHR22972:SF7
PDB ID(s) N.A.,
pfam ID PF00069,
Drug Bank ID N.A.,
ChEMBL ID CHEMBL3337330
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Japanese encephalitis virus
Virus Short Name JEV
Order Amarillovirales
Virus Family Flaviviridae
Virus Subfamily N.A.
Genus Flavivirus
Species Japanese encephalitis virus
Host Vertebrates
Cell Tropism
Associated Disease Encephalitis
Mode of Transmission Sexual contact, blood, breast feeding
VIPR DB link https://www.viprbrc.org/brc/vipr_search.do?species=Japanese_encephalitis_virus
ICTV DB link https://ictv.global/report/183/flaviviridae
Virus Host DB link

Publication Information

Paper Title C19orf66 Inhibits Japanese Encephalitis Virus Replication by Targeting -1 PRF and the NS3 Protein
Author's Name Du Yu 1, Yundi Zhao 1, Junhui Pan 1, Xingmiao Yang 1, Zhenjie Liang 1, Shengda Xie 1, Ruibing Cao 2
Journal Name VIROLOGICA SINICA
Pubmed ID 34309824
Abstract The Japanese encephalitis serogroup of the neurogenic Flavivirus has a specific feature that expresses a non-structural protein NS1' produced through a programmed -1 ribosomal frameshifting (-1 PRF). Herein, C19orf66, a novel member of interferon-stimulated gene (ISG) products, exhibited significant activity of antagonizing Japanese encephalitis virus (JEV) infection. Overexpression of C19orf66 in 293T cells significantly inhibited JEV replication, while knock-down of endogenous C19orf66 in HeLa cells and A549 cells significantly increased virus replication. Notably, C19orf66 had an inhibitory effect on frameshift production of JEV NS1'. The inhibition was more significant when C19orf66 and JEV NS1-NS2A were co-expressed in the 293T cells. Both C19orf66-209 and C19orf66-Zincmut did not significantly change the NS1' to NS1 ratio and had weaker antiviral effects than C19orf66. Similarly, C19orf66-209 and C19orf66-Zincmut had no significant effect on the expression of the JEV NS3 protein, whose expression was down-regulated by C19orf66 via the lysosome-dependent pathway. These findings suggest that C19orf66 may possess at least two different mechanisms of antagonizing JEV infection. This study identified C19orf66 as a novel interferon-stimulated gene product that can inhibit JEV replication by targeting -1 PRF and the NS3 protein. The study provides baseline information for the future development of broad-spectrum antiviral agents against JEV.
Used Model 293T,HELA,A549
DOI 10.1007/s12250-021-00423-6