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Virus Details


VHFID10107

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name TLR4
HF Protein Name Toll-Like Receptor
HF Function
Uniprot ID O00206
Protein Sequence View Fasta Sequence
NCBI Gene ID 7099
Host Factor (HF) Name in Paper TLR4
Gene synonyms N.A.
Ensemble Gene ID ENSG00000136869
Ensemble Transcript ENST00000355622.8 [O00206-1];ENST00000394487.5 [O00206-2]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0001530, GO:0001540, GO:0001726, GO:0001875, GO:0001891, GO:0002224, GO:0002246, GO:0002322, GO:0002537, GO:0002730, GO:0002755, GO:0004888, GO:0005102, GO:0005737, GO:0005769, GO:0005886, GO:0006809, GO:0006909, GO:0006954, GO:0006955, GO:0007252, GO:0007254, GO:0009897, GO:0009986, GO:0010008, GO:0010467, GO:0010572, GO:0010628, GO:0014002, GO:0014004, GO:0014911, GO:0016046, GO:0030890, GO:0031663, GO:0032496, GO:0032497, GO:0032689, GO:0032700, GO:0032707, GO:0032715, GO:0032720, GO:0032722, GO:0032727, GO:0032728, GO:0032729, GO:0032731, GO:0032732, GO:0032733, GO:0032735, GO:0032755, GO:0032757, GO:0032760, GO:0032874, GO:0034142, GO:0035666, GO:0036120, GO:0038023, GO:0042088, GO:0042116, GO:0042742, GO:0042802, GO:0043032, GO:0043123, GO:0043235, GO:0043406, GO:0045087, GO:0045342, GO:0045348, GO:0045429, GO:0045671, GO:0045944, GO:0046330, GO:0046696, GO:0046982, GO:0048471, GO:0048661, GO:0050729, GO:0050829, GO:0051092, GO:0051403, GO:0051770, GO:0060729, GO:0060907, GO:0061809, GO:0070371, GO:0070373, GO:0070374, GO:0070427, GO:0070430, GO:0070431, GO:0070434, GO:0071222, GO:0071223, GO:0071260, GO:0071346, GO:0120163, GO:0140052, GO:1900017, GO:1900227, GO:1901224, GO:1903428, GO:1903974, GO:1904466, GO:1904646, GO:2000343, GO:2001238,
MINT ID O00206
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 603030
PANTHER ID PTHR24365;PTHR24365:SF521
PDB ID(s) 2Z62, 2Z63, 2Z65, 2Z66, 3FXI, 3UL7, 3UL8, 3UL9, 3ULA, 4G8A, 5NAM, 5NAO, 8WO1, 8WTA,
pfam ID PF12799, PF13516, PF13855, PF01582,
Drug Bank ID DB02767, DB00924, DB06447, DB04933, DB05475, DB03017, DB13615, DB08231, DB01183, DB11193,
ChEMBL ID CHEMBL5255
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Japanese encephalitis virus
Virus Short Name JEV
Order Amarillovirales
Virus Family Flaviviridae
Virus Subfamily N.A.
Genus Flavivirus
Species Japanese encephalitis virus
Host Vertebrates
Cell Tropism
Associated Disease Encephalitis
Mode of Transmission Sexual contact, blood, breast feeding
VIPR DB link https://www.viprbrc.org/brc/vipr_search.do?species=Japanese_encephalitis_virus
ICTV DB link https://ictv.global/report/183/flaviviridae
Virus Host DB link

Publication Information

Paper Title Proteomic analyses identify intracellular targets for Japanese encephalitis virus nonstructural protein 1 (NS1)
Author's Name Peng Wang 1, Xinze Liu 1, Qi Li 1, Jue Wang 1, Wenke Ruan 4
Journal Name Virus Research
Pubmed ID 34175344
Abstract Japanese encephalitis is a zoonotic disease caused by Japanese encephalitis virus (JEV). JEV nonstructural protein 1 (NS1) is involved in many crucial biological events during viral infection and immune suppression. To investigate the role of JEV NS1 in virus-infected cells, the molecules with which it interacts intracellularly were screened with a pull-down assay and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The interaction between heterogeneous nuclear ribonucleoprotein K (hnRNP K), vimentin and NS1 were verified with coimmunoprecipitation and confocal assays. Our results show that JEV NS1 interacts with vimentin, hnRNP K and colocalizes with cellular vimentin and hnRNP K. Furthermore, our results demonstrate that the expression of vimentin and hnRNP K were up-regulated in both NS1-transfected and JEV-infected cells. Knocking down vimentin and hnRNP K reduced viral replication while conversely, over-expression of vimentin and hnRNP K improved viral replication, suggesting an important role for this protein in the viral life cycle. Also, We found that vimentin also interacted with hnRNP K after overexpression of NS1 or JEV infection. These findings provide insight into the molecular mechanism of JEV replication and highlight the key role the NS1 in JEV infection.
Used Model Bhk 21 and pk17
DOI 10.1016/j.virusres.2021.198497