| Gene Name | HSPA5 |
| HF Protein Name | Endoplasmic reticulum chaperone BiP |
| HF Function | Essential for viral entry |
| Uniprot ID | Q90593 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 396487 |
| Host Factor (HF) Name in Paper | GRP78 |
| Gene synonyms | GRP78 |
| Ensemble Gene ID | ENSGALG00000001000 |
| Ensemble Transcript | ENSGALT00000001476 |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0005524, GO:0005788, GO:0016887, GO:0090074, GO:1903895, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | N.A. |
| PANTHER ID | PTHR19375 |
| PDB ID(s) | N.A., |
| pfam ID | PF00012, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Gallus gallus (Chicken) |
| Virus Name | Avian leukosis virus |
| Virus Short Name | ALV |
| Order | Unassigned |
| Virus Family | Retroviridae |
| Virus Subfamily | Orthoretrovirinae |
| Genus | Alpharetrovirus |
| Species | Avian leukosis virus |
| Host | Vertebrates |
| Cell Tropism | N.A. |
| Associated Disease | Malignancies |
| Mode of Transmission | N.A. |
| VIPR DB link | N.A. |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/reverse-transcribing-dna-and-rna-viruses-2011/w/rt_viruses/161/retroviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Retroviridae |
| Paper Title | Membrane associated GRP78 helps subgroup J avian leucosis virus enter cells |
| Author's Name | Lin Wang, Mei Mei, Aijian Qin, Jianqiang Ye, Kun Qian and Hongxia Shao |
| Journal Name | Veterinary Research |
| Pubmed ID | 27599847 |
| Abstract | We previously identified chicken Annexin A2 (chANXA2) as a novel receptor for retrovirus avian leucosis virus subgroup J (ALV-J), using a DF1 cell line expressing the viral envelope (env) protein. To further probe whether other proteins participate in virus infection, we investigated several host proteins from co-immunoprecipitation with the DF1 cell line expressing viral env. Mass spectrometry analysis indicates that the chicken glucose-regulation protein 78 (chGRP78) of the DF1 membrane interacted with the ALV-J env protein. The results revealed that antibodies or siRNA to chGRP78 significantly inhibited ALV-J infection and replication, and over-expression of chGRP78 enabled the entry of ALV-J into non-susceptible cells. Taken together, these results are the first to report that chGRP78 functions to help ALV-J enter cells. |
| Used Model | DF1, HEK293T and GEF cells |
| DOI | 10.1186/s13567-016-0373-6 |
