Hostfactor - VHFIDB

Gene Name	RAB5A
HF Protein Name	Ras-related protein Rab-5A
HF Function	Involved in Borna Disease Virus cell entry
Uniprot ID	P20339
Protein Sequence	View Fasta Sequence
NCBI Gene ID	5868
Host Factor (HF) Name in Paper	Rab5
Gene synonyms	RAB5
Ensemble Gene ID	ENSG00000144566
Ensemble Transcript	ENST00000273047 [P20339-1];ENST00000422242 [P20339-2]
KEGG ID	Go to KEGG Database
Gene Ontology ID(s)	GO:0001726, GO:0003924, GO:0005525, GO:0005737, GO:0005768, GO:0005769, GO:0005829, GO:0005886, GO:0006661, GO:0006897, GO:0006909, GO:0007596, GO:0008021, GO:0010008, GO:0015031, GO:0015629, GO:0019003, GO:0030100, GO:0030139, GO:0030424, GO:0030425, GO:0030665, GO:0030670, GO:0031901, GO:0036465, GO:0036477, GO:0039694, GO:0042470, GO:0043025, GO:0043195, GO:0043679, GO:0043687, GO:0045022, GO:0045056, GO:0045121, GO:0045921, GO:0051036, GO:0051489, GO:0061024, GO:0070062, GO:0098559, GO:2000286, GO:2000300, GO:2000785,
MINT ID	P20339
STRING	Click to see interaction map
GWAS Analysis	Click to see gwas analysis
OMIM ID	179512
PANTHER ID	N.A.
PDB ID(s)	1N6H, 1N6I, 1N6K, 1N6L, 1N6N, 1N6O, 1N6P, 1N6R, 1R2Q, 1TU3, 1TU4, 3MJH, 4Q9U,
pfam ID	PF00071,
Drug Bank ID	DB04315, DB04137, DB02467,
ChEMBL ID	N.A.
Organism	Homo sapiens (Human)

Virus Name	Borna disease virus
Virus Short Name	BDV
Order	Mononegavirales
Virus Family	Bornaviridae
Virus Subfamily	N.A.
Genus	Bornavirus
Species	Borna disease virus
Host	Horses, sheep, cattle, rodents, birds, sometimes human also
Cell Tropism	Neurons and astrocytes, also infect oligodendrocytes and ependymal cells
Associated Disease	Borna disease ( in mammals), encephalitis, proventricular dilatation disease (in birds)
Mode of Transmission	Direct contact with salivary, urine and feces
VIPR DB link	N.A.
ICTV DB link	https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/195/bornaviridae
Virus Host DB link	N.A.

Paper Title	Cell Entry of Borna disease virus Follows a clathrin-Mediated endocytosis pathway that requires Rab5 and Microtubules
Author's Name	Roberto Clemente and Juan C. de la Torre
Journal Name	Journal Of Virology
Pubmed ID	19656886
Abstract	Borna disease virus (BDV), the prototypic member of the Bornaviridae family within the order Mononegavirales, exhibits high neurotropism and provides an important and unique experimental model system for studying virus-cell interactions within the central nervous system. BDV surface glycoprotein (G) plays a critical role in virus cell entry via receptor-mediated endocytosis, and therefore, G is a critical determinant of virus tissue and cell tropism. However, the specific cell pathways involved in BDV cell entry have not been determined. Here, we provide evidence that BDV uses a clathrin-mediated, caveola-independent cell entry pathway. We also show that BDV G-mediated fusion takes place at an optimal pH of 6.0 to 6.2, corresponding to an early-endosome compartment. Consistent with this finding, BDV cell entry was Rab5 dependent but Rab7 independent and exhibited rapid fusion kinetics. Our results also uncovered a key role for microtubules in BDV cell entry, whereas the integrity and dynamics of actin cytoskeleton were not required for efficient cell entry of BDV.
Used Model	Ol and Vero E6 cells
DOI	10.1128/JVI.00990-09

Virus Details