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Virus Details


VHFID3099

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name CYP1A2
HF Protein Name Cytochrome P450 1A2
HF Function Inhibits Poliovirus replication
Uniprot ID P05177
Protein Sequence View Fasta Sequence
NCBI Gene ID 1544
Host Factor (HF) Name in Paper CYP1A2
Gene synonyms N.A.
Ensemble Gene ID ENSG00000140505
Ensemble Transcript ENST00000343932 [P05177-2]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0004497, GO:0005506, GO:0005789, GO:0006706, GO:0006778, GO:0006805, GO:0009055, GO:0009403, GO:0009791, GO:0009820, GO:0010468, GO:0016098, GO:0016491, GO:0016712, GO:0017144, GO:0018894, GO:0019373, GO:0019825, GO:0019899, GO:0020037, GO:0030324, GO:0031090, GO:0032259, GO:0032355, GO:0032451, GO:0032496, GO:0032787, GO:0034875, GO:0035902, GO:0042737, GO:0042738, GO:0043231, GO:0045333, GO:0046483, GO:0050665, GO:0055114, GO:0070330, GO:0070989, GO:0071276, GO:0071280, GO:0071615, GO:0097267,
MINT ID N.A.
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 108330
PANTHER ID N.A.
PDB ID(s) 2HI4,
pfam ID PF00067,
Drug Bank ID DB07453, DB05812, DB01418, DB00316, DB00787, DB06594, DB00518, DB00523, DB00918, DB00969, DB00357, DB01424, DB01223, DB01118, DB00321, DB00381, DB00261, DB01217, DB01435, DB06605, DB00714, DB00673, DB06413, DB06216, DB01072, DB00572, DB06626, DB00972, DB00207, DB06769, DB06770, DB06732, DB00195, DB00188, DB01558, DB01200, DB00297, DB00921, DB01156, DB00201, DB06774, DB00564, DB00389, DB00262, DB01136, DB00477, DB00356, DB01166, DB00501, DB01012, DB00568, DB00537, DB00604, DB00215, DB01211, DB01407, DB04920, DB01013, DB00882, DB01242, DB00575, DB00758, DB00257, DB00363, DB00286, DB05219, DB00924, DB00851, DB04816, DB06292, DB01254, DB00694, DB00705, DB01151, DB00967, DB01191, DB00633, DB00829, DB00586, DB00917, DB01075, DB00822, DB01184, DB00988, DB01142, DB00476, DB00974, DB00625, DB06210, DB00467, DB00494, DB00668, DB00696, DB00530, DB00199, DB00783, DB00655, DB04574, DB00898, DB00773, DB01628, DB00196, DB04841, DB01544, DB00544, DB00472, DB00623, DB00499, DB01095, DB00176, DB00998, DB01241, DB01645, DB08909, DB00365, DB00400, DB05708, DB00629, DB00502, DB01094, DB01355, DB11737, DB04946, DB00619, DB00458, DB00724, DB01306, DB09456, DB01307, DB00047, DB01309, DB00030, DB00046, DB00071, DB00033, DB01029, DB00951, DB09570, DB01026, DB00448, DB01097, DB01002, DB01137, DB00281, DB00978, DB01601, DB04871, DB00678, DB01283, DB00772, DB00934, DB00737, DB01065, DB00170, DB00532, DB01357, DB00333, DB00763, DB01403, DB00553, DB01028, DB00379, DB06148, DB01388, DB01110, DB00834, DB00370, DB01171, DB00745, DB00461, DB00607, DB00788, DB01149, DB00220, DB00238, DB06803, DB00184, DB01115, DB06712, DB00401, DB00435, DB00325, DB00368, DB01059, DB00540, DB05990, DB01165, DB00334, DB00338, DB00904, DB01173, DB09330, DB00526, DB01303, DB06412, DB00377, DB00213, DB00715, DB06589, DB00487, DB00008, DB00022, DB00738, DB00806, DB00850, DB03783, DB01174, DB00191, DB00388, DB00397, DB01100, DB01621, DB08910, DB01058, DB01087, DB00794, DB00396, DB01131, DB00420, DB01182, DB00818, DB00571, DB00339, DB00908, DB00468, DB01129, DB00980, DB00863, DB01367, DB02709, DB00615, DB01045, DB08864, DB00740, DB00503, DB00953, DB00533, DB00268, DB00296, DB00412, DB05271, DB00778, DB12332, DB00418, DB01037, DB01104, DB06290, DB06819, DB00398, DB00428, DB06820, DB00605, DB00382, DB00675, DB01079, DB00976, DB01405, DB00300, DB00857, DB01041, DB01412, DB00277, DB00730, DB00679, DB01623, DB00208, DB01007, DB00697, DB01056, DB00539, DB00752, DB00384, DB00831, DB08867, DB00577, DB00313, DB08881, DB00661, DB00682, DB00549, DB00744, DB00246, DB00315, DB00425,
ChEMBL ID CHEMBL3356
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Polio Virus
Virus Short Name PV
Order Picornavirales
Virus Family Picornaviridae
Virus Subfamily N.A.
Genus Enterovirus
Species Enterovirus C
Host Vertebrates
Cell Tropism The gastrointestinal tract
Associated Disease Poliomyelitis
Mode of Transmission Sexual contact, blood, breast feeding
VIPR DB link https://www.viprbrc.org/brc/home.spg?decorator=picorna
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/234/picornaviridae
Virus Host DB link http://www.genome.jp/virushostdb/view/?virus_lineage=Picornaviridae

Publication Information

Paper Title Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game
Author's Name Sabine M. G. van der Sanden, Weilin Wu, Naomi Dybdahl-Sissoko, William C. Weldon, Paula Brooks,bJason O'Donnell, Les P. Jones, Cedric Brown, S. Mark Tompkins, M. Steven Oberste,corresponding authora Jon Karpilow and Ralph A. Tripp
Journal Name Journal Of Virology
Pubmed ID 26581994
Abstract Vaccine manufacturing costs prevent a significant portion of the world's population from accessing protection from vaccine-preventable diseases. To enhance vaccine production at reduced costs, a genome-wide RNA interference (RNAi) screen was performed to identify gene knockdown events that enhanced poliovirus replication. Primary screen hits were validated in a Vero vaccine manufacturing cell line using attenuated and wild-type poliovirus strains. Multiple single and dual gene silencing events increased poliovirus titers >20-fold and >50-fold, respectively. Host gene knockdown events did not affect virus antigenicity, and clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9-mediated knockout of the top candidates dramatically improved viral vaccine strain production. Interestingly, silencing of several genes that enhanced poliovirus replication also enhanced replication of enterovirus 71, a clinically relevant virus to which vaccines are being targeted. The discovery that host gene modulation can markedly increase virus vaccine production dramatically alters mammalian cell-based vaccine manufacturing possibilities and should facilitate polio eradication using the inactivated poliovirus vaccine. IMPORTANCE: Using a genome-wide RNAi screen, a collection of host virus resistance genes was identified that, upon silencing, increased poliovirus and enterovirus 71 production by from 10-fold to >50-fold in a Vero vaccine manufacturing cell line. This report provides novel insights into enterovirus-host interactions and describes an approach to developing the next generation of vaccine manufacturing through engineered vaccine cell lines. The results show that specific gene silencing and knockout events can enhance viral titers of both attenuated (Sabin strain) and wild-type polioviruses, a finding that should greatly facilitate global implementation of inactivated polio vaccine as well as further reduce costs for live-attenuated oral polio vaccines. This work describes a platform-enabling technology applicable to most vaccine-preventable diseases.
Used Model HEp-2C and Vero cells
DOI 10.1128/JVI.01464-15