Download Help

Virus Details


VHFID3207

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name DDX3X
HF Protein Name ATP-dependent RNA helicase DDX3X
HF Function Restricts Hepatitis B Virus Replication at the transcriptional level
Uniprot ID O00571
Protein Sequence View Fasta Sequence
NCBI Gene ID 1654
Host Factor (HF) Name in Paper DDX3
Gene synonyms DBX DDX3
Ensemble Gene ID ENSG00000215301
Ensemble Transcript ENST00000399959 [O00571-1];ENST00000457138 [O00571-2];ENST00000478993 [O00571-1];ENST00000629496 [O00571-1];ENST00000629785 [O00571-1];ENST00000630255 [O00571-1]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0003677, GO:0003723, GO:0003924, GO:0004003, GO:0004004, GO:0005524, GO:0005576, GO:0005634, GO:0005730, GO:0005737, GO:0005741, GO:0005829, GO:0006351, GO:0006413, GO:0007059, GO:0008134, GO:0008143, GO:0008190, GO:0008625, GO:0009615, GO:0010494, GO:0010501, GO:0010628, GO:0016032, GO:0016055, GO:0016607, GO:0016887, GO:0017111, GO:0017148, GO:0030307, GO:0030308, GO:0031333, GO:0031369, GO:0032728, GO:0033592, GO:0034063, GO:0034774, GO:0035556, GO:0035613, GO:0042256, GO:0043024, GO:0043065, GO:0043066, GO:0043154, GO:0043273, GO:0043280, GO:0043312, GO:0043539, GO:0045070, GO:0045087, GO:0045296, GO:0045727, GO:0045944, GO:0045948, GO:0048027, GO:0070062, GO:0071243, GO:0071470, GO:0071651, GO:0071902, GO:0090263, GO:0097193, GO:1900087, GO:1903608, GO:1904813, GO:2001243,
MINT ID O00571
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 300160
PANTHER ID N.A.
PDB ID(s) 2I4I, 2JGN, 3JRV, 4O2C, 4O2E, 4O2F, 4PX9, 4PXA, 5E7I, 5E7J, 5E7M,
pfam ID PF00270, PF00271,
Drug Bank ID N.A.,
ChEMBL ID CHEMBL5553
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Hepatitis B virus
Virus Short Name HBV
Order Unassigned
Virus Family Hepadnaviridae
Virus Subfamily N.A.
Genus Orthohepadnavirus
Species Hepatitis B virus
Host Human, mammals
Cell Tropism Hepatocytes
Associated Disease Hepatitis, hepatocellular carcinoma(chronic infections), cirrhosis
Mode of Transmission Sexual contact, blood, maternal-neonatal
VIPR DB link N.A.
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_9th_report/reverse-transcribing-dna-and-rna-viruses-2011/w/rt_viruses/155/hepadnaviridae
Virus Host DB link N.A.

Publication Information

Paper Title DDX3 DEAD-box RNA helicase is a host factor that restricts hepatitis B virus replication at the transcriptional level
Author's Name Chunkyu Ko, Sooyoung Lee, Marc P. Windisch, Wang-Shick Ryu
Journal Name Journal Of Virology
Pubmed ID 25231298
Abstract DDX3 is a member of the DEAD-box RNA helicase family, involved in mRNA metabolism, including transcription, splicing, and translation. We previously identified DDX3 as a hepatitis B virus (HBV) polymerase (Pol) binding protein, and by using a transient transfection, we found that DDX3 inhibits HBV replication at the posttranscriptional level, perhaps following encapsidation. To determine the exact mechanism of the inhibition, we here employed a diverse HBV experimental system. Inconsistently, we found that DDX3-mediated inhibition occurs at the level of transcription. By using tetracycline-inducible HBV-producing cells, we observed that lentivirus-mediated DDX3 expression led to a reduced level of HBV RNAs. Importantly, knockdown of DDX3 by short hairpin RNA resulted in augmentation of HBV RNAs in two distinct HBV replication systems: (i) tetracycline-inducible HBV-producing cells and (ii) constitutive HBV-producing HepG2.2.15 cells. Moreover, DDX3 knockdown in HBV-susceptible HepG2-NTCP cells, where covalently closed circular DNA (cccDNA) serves as the template for viral transcription, resulted in increased HBV RNAs, validating that transcription regulation by DDX3 occurs on a physiological template. Overall, our results demonstrate that DDX3 represents an intrinsic host antiviral factor that restricts HBV transcription.
Used Model HepG2 cells
DOI 10.1128/JVI.02035-14