| Gene Name | PTBP1 |
| HF Protein Name | Polypyrimidine tract-binding protein 1 |
| HF Function | Required for HAstV replication |
| Uniprot ID | P26599 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 5725 |
| Host Factor (HF) Name in Paper | hnRNP1 |
| Gene synonyms | PTB |
| Ensemble Gene ID | ENSG00000011304 |
| Ensemble Transcript | ENST00000349038 [P26599-1];ENST00000356948 [P26599-3];ENST00000394601 [P26599-2] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000381, GO:0000398, GO:0003723, GO:0005654, GO:0005730, GO:0006397, GO:0008187, GO:0008380, GO:0008543, GO:0016020, GO:0016070, GO:0033119, GO:0036002, GO:0048025, GO:0051148, GO:0070062, GO:0075522, |
| MINT ID | P26599 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 600693 |
| PANTHER ID | N.A. |
| PDB ID(s) | 1QM9, 1SJQ, 1SJR, 2AD9, 2ADB, 2ADC, 2EVZ, 2N3O, 3ZZY, 3ZZZ, |
| pfam ID | PF00076, |
| Drug Bank ID | N.A., |
| ChEMBL ID | CHEMBL1293230 |
| Organism | Homo sapiens (Human) |
| Virus Name | Human astrovirus |
| Virus Short Name | HAstV |
| Order | Nidovirales |
| Virus Family | Astroviridae |
| Virus Subfamily | N.A. |
| Genus | Mamastrovirus |
| Species | Human astrovirus |
| Host | Human, mammals |
| Cell Tropism | Enterocytes |
| Associated Disease | Infantile gastroenteritis |
| Mode of Transmission | Fecal-oral |
| VIPR DB link | N.A. |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/247/astroviridae |
| Virus Host DB link | N.A. |
| Paper Title | PTB Binds to the 3 Untranslated Region of the Human Astrovirus Type 8: A Possible Role in Viral Replication |
| Author's Name | Wendy Espinosa-Hernandez, Dora Velez-Uriza, Jesus Valdes, Cristina Velez-Del Valle, Juan Salas- Benito, Rebeca Martnez-Contreras, Matilde Garca-Esptia, Mariana Salas-Benito, Tania Vega- Almeida, Monica De Nova-Ocampo |
| Journal Name | plos one |
| Pubmed ID | 25406089 |
| Abstract | The 3 untranslated region (3UTR) of human astroviruses (HAstV) consists of two hairpin structures (helix I and II) joined by a linker harboring a conserved PTB/hnRNP1 binding site. The identification and characterization of cellular proteins that interact with the 3 UTR of HAstV-8 virus will help to uncover cellular requirements for viral functions. To this end, mobility shift assays and UV cross-linking were performed with uninfected and HAstV-8-infected cell extracts and HAstV-8 3UTR probes. Two RNA-protein complexes (CI and CII) were recruited into the 3 UTR. Complex CII formation was compromised with cold homologous RNA, and seven proteins of 35, 40, 45, 50, 52, 57/60 and 75 kDa were cross-linked to the 3 UTR. Supermobility shift assays indicated that PTB/hnRNP1 is part of this complex, and 3 UTR-crosslinked PTB/hnRNP1 was immunoprecipitated from HAstV-8 infected cell-membrane extracts. Also, immunofluorescence analyses revealed that PTB/hnRNP1 is distributed in the nucleus and |
| Used Model | Caco-2 cells |
| DOI | 10.1371/journal.pone.0113113 |
