| Gene Name | IRF3 |
| HF Protein Name | Interferon regulatory factor 3 |
| HF Function | Activates interferon-beta after virus infection |
| Uniprot ID | Q14653 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 3661 |
| Host Factor (HF) Name in Paper | IRF3 |
| Gene synonyms | N.A. |
| Ensemble Gene ID | ENSG00000126456 |
| Ensemble Transcript | ENST00000309877 [Q14653-1];ENST00000377139 [Q14653-1];ENST00000442265 [Q14653-5];ENST00000596765 [Q14653-3];ENST00000597198 [Q14653-1];ENST00000599223 [Q14653-2];ENST00000600022 [Q14653-3];ENST00000601291 [Q14653-4] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000978, GO:0000981, GO:0001077, GO:0001078, GO:0001228, GO:0003677, GO:0003712, GO:0005634, GO:0005654, GO:0005737, GO:0005829, GO:0006366, GO:0006915, GO:0006974, GO:0016032, GO:0019904, GO:0031663, GO:0032479, GO:0032480, GO:0032481, GO:0032727, GO:0032728, GO:0035666, GO:0039530, GO:0042802, GO:0042803, GO:0043123, GO:0043330, GO:0043565, GO:0045351, GO:0045358, GO:0045944, GO:0050689, GO:0050715, GO:0051607, GO:0060333, GO:0060337, GO:0060340, GO:0071359, GO:0071888, GO:0097300, |
| MINT ID | Q14653 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 603734 |
| PANTHER ID | PTHR11949 |
| PDB ID(s) | 1J2F, 1QWT, 1T2K, 1ZOQ, 2O61, 2O6G, 2PI0, 3A77, 3QU6, 5JEJ, 5JEK, 5JEL, 5JEM, 5JEO, 5JER, |
| pfam ID | PF00605, PF10401, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Sendai virus |
| Virus Short Name | SeV |
| Order | Mononegavirales |
| Virus Family | Paramyxoviridae |
| Virus Subfamily | N.A. |
| Genus | Respirovirus |
| Species | Murine respirovirus |
| Host | Mammals |
| Cell Tropism | N.A. |
| Associated Disease | Acute febrile respiratory tract infection |
| Mode of Transmission | Respiratory |
| VIPR DB link | http://www.viprbrc.org/brc/vipr_allSpecies_search.do?method=SubmitForm&decorator=paramyxo |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/199/paramyxoviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Paramyxoviridae |
| Paper Title | Genome-wide RNAi screen reveals a new role of a WNT/ CTNNB1 signaling pathway as negative regulator of virus-induced innate immune responses |
| Author's Name | Martin Baril, Salwa Es-Saad, Laurent Chatel-Chaix, Karin Fink, Tram Pham, Vale rie-Ann Raymond, Karine Audette, Anne-Sophie Guenier, Jean Duchaine, Marc Servant, Marc Bilodeau, E ric Cohen, Nathalie Grandvaux, Daniel Lamarre |
| Journal Name | PLOS Pathogens |
| Pubmed ID | 23785285 |
| Abstract | To identify new regulators of antiviral innate immunity, we completed the first genome-wide gene silencing screen assessing the transcriptional response at the interferon-beta (IFNB1) promoter following Sendai virus (SeV) infection. We now report a novel link between WNT signaling pathway and the modulation of retinoic acid-inducible gene I (RIG-I)-like receptor (RLR)-dependent innate immune responses. Here we show that secretion of WNT2B and WNT9B and stabilization of beta-catenin (CTNNB1) upon virus infection negatively regulate expression of representative inducible genes IFNB1, IFIT1 and TNF in a CTNNB1-dependent effector mechanism. The antiviral response is drastically reduced by glycogen synthase kinase 3 (GSK3) inhibitors but restored in CTNNB1 knockdown cells. The findings confirm a novel regulation of antiviral innate immunity by a canonical-like WNT/CTNNB1 signaling pathway. The study identifies novel avenues for broad-spectrum antiviral targets and preventing immune-mediated diseases upon viral infection. |
| Used Model | HEK 293T and A549 cells |
| DOI | 10.1371/journal.ppat.1003416 |
