| Gene Name | CH25H |
| HF Protein Name | Cholesterol 25-hydroxylase |
| HF Function | Inhibits virus entry |
| Uniprot ID | F1SCY5 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 100157868 |
| Host Factor (HF) Name in Paper | CH25H |
| Gene synonyms | N.A. |
| Ensemble Gene ID | ENSSSCG00000010449 |
| Ensemble Transcript | ENSSSCT00000011435 |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0005506, GO:0008610, GO:0016021, GO:0016491, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | N.A. |
| PANTHER ID | N.A. |
| PDB ID(s) | N.A., |
| pfam ID | PF04116, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Sus scrofa (Pig) |
| Virus Name | Porcine reproductive and respiratory syndrome virus |
| Virus Short Name | PRRSV |
| Order | Nidovirales |
| Virus Family | Arteriviridae |
| Virus Subfamily | N.A. |
| Genus | Porartevirus |
| Species | Porcine reproductive and respiratory syndrome virus |
| Host | Vertebrates |
| Cell Tropism | Primarily it's found in alveolar lung macrophages and macrophages of other tissues, later, is also found in testicular germ cells. |
| Associated Disease | Abortions and respiratory disease |
| Mode of Transmission | Transplacental, saliva, urine, semen and respiratory tract secretions |
| VIPR DB link | N.A. |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/220/arteriviridae |
| Virus Host DB link | N.A. |
| Paper Title | Cholesterol 25-Hydroxylase is an Interferon-inducible Factor that Protects against Porcine Reproductive and Respiratory Syndrome Virus Infection |
| Author's Name | Zhongbao Song, Qiaoya Zhang, Xuewei Liu, Juan Bai, Yongxiang Zhao, Xianwei Wang, Ping Jiang |
| Journal Name | Veterinary Microbiology |
| Pubmed ID | 29103685 |
| Abstract | Porcine reproductive and respiratory syndrome virus (PRRSV), a single-stranded, positive-sense RNA virus of the Arteriviridae family, has become a global health threat for swine. Cholesterol 25-hydroxylase (CH25H) is an enzyme that catalyzes oxidation of cholesterolto 25-hydroxycholesterol (25HC). The purpose of this study was to explore the antiviral activity of CH25H against PRRSV infection. We found that CH25H was induced by interferon-alpha and PRRSV in Marc-145 monkey kidney cells. In addition, CH25H and 25HC significantly inhibited PRRSV infection by preventing virus entry. A CH25H mutant that exhibited decreased catalytic activity had an antiviral effect against PRRSV. Treatment with 25HC pre-infection or post-infection significantly inhibited PRRSV infection in primary porcine alveolar macrophages. Our results reveal that CH25H is an interferon-stimulated gene and its production of 25HC can be used as a natural antiviral agent to combat PRRSV infection. |
| Used Model | Marc-145 cells |
| DOI | 10.1016/j.vetmic.2017.09.011 |
