| Gene Name | TRIM14 |
| HF Protein Name | Tripartite motif-containing protein 14 |
| HF Function | Suppresses SINV virus reproduction |
| Uniprot ID | Q14142 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 9830 |
| Host Factor (HF) Name in Paper | TRIM14 |
| Gene synonyms | KIAA0129 |
| Ensemble Gene ID | ENSG00000106785 |
| Ensemble Transcript | ENST00000341469 [Q14142-1];ENST00000342043 [Q14142-1];ENST00000375098 [Q14142-1] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0005737, GO:0005741, GO:0008270, GO:0032897, GO:0045087, GO:0051091, GO:0051092, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 606556 |
| PANTHER ID | N.A. |
| PDB ID(s) | N.A., |
| pfam ID | PF13765, PF00622, PF00643, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Sindbis virus |
| Virus Short Name | SINV |
| Order | Unassigned |
| Virus Family | Togaviridae |
| Virus Subfamily | N.A. |
| Genus | Alphavirus |
| Species | Sindbis virus |
| Host | Human, mammals,mosquitoes and birds |
| Cell Tropism | N.A. |
| Associated Disease | Sindbis fever |
| Mode of Transmission | By infected mosquito |
| VIPR DB link | https://www.viprbrc.org/brc/vipr_allSpecies_search.spg?method=SubmitForm&decorator=toga |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/275/togaviridae |
| Virus Host DB link | N.A. |
| Paper Title | Enhanced expression of trim14 gene suppressed Sindbis virus reproduction and modulated the transcription of a large number of genes of innate immunity |
| Author's Name | Nenasheva VV, Kovaleva GV, Uryvaev LV, Ionova KS, Dedova AV, Vorkunova GK, Chernyshenko SV, Khaidarova NV, Tarantul VZ. |
| Journal Name | Immunologic Research |
| Pubmed ID | 25948474 |
| Abstract | In the present research, we have studied an influence of enhanced expression TRIM14 on alphavirus Sindbis (SINV, Togaviridae family) infection. In the HEK293 cells transfected with human trim14 gene (HEK-trim14), SINV yield after infection was decreased 1000-10,000 times (3-4 lg of TCD50/ml) at 24 h p.i. and considerably less (1-2 lg of TCD50/ml) at 48 h p.i. Analysis of the expression of 43 genesdirectly or indirectly involved in innate immune machine in HEK-trim14 non-infected cells comparing with the control (non-transfected) HEK293 cells revealed that stable trim14 transfection in HEK293 cells caused increased transcription of 18 genes (ifna, il6 (ifnbeta2), isg15, raf-1, NF-kB (nf-kb1, rela, nf-kb2, relb), grb2, grb3-3, traf3ip2, junB, c-myb, pu.1, akt1, tyk2, erk2, mek2) and lowered transcription of 3 genes (ifngamma, gata1, il-17a). The similar patterns of genes expression observe in SINV-infected non-transfected HEK293 cells. However, SINV infection of HEK-trim14 cells caused inhibition of the most interferon cascade genes as well as subunits of transcription factor NF-κB. Thus, stable enhanced expression of trim14 gene in cells activates the transcription of many immunity genesand suppresses the SINV reproduction, but SINV infection of HEK-trim14 cells promotes inhibition of some genes involved in innate immune system. |
| Used Model | HEK293 cells |
| DOI | 10.1007/s12026-015-8653-1 |
