| Gene Name | TIMD4 |
| HF Protein Name | T-cell immunoglobulin and mucin domain-containing protein 4 |
| HF Function | Required for virus entry |
| Uniprot ID | Q96H15 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 91937 |
| Host Factor (HF) Name in Paper | TIM4 |
| Gene synonyms | TIM4 |
| Ensemble Gene ID | ENSG00000145850 |
| Ensemble Transcript | ENST00000274532 [Q96H15-1];ENST00000407087 [Q96H15-2] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0016021, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 610096 |
| PANTHER ID | N.A. |
| PDB ID(s) | 5DZN, 5F7F, 5F7H, |
| pfam ID | PF07686, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Zaire ebolavirus |
| Virus Short Name | ZEBOV |
| Order | Bunyavirales |
| Virus Family | Filoviridae |
| Virus Subfamily | N.A. |
| Genus | Ebolavirus |
| Species | Zaire ebolavirus |
| Host | Bats, human and primates |
| Cell Tropism | N.A. |
| Associated Disease | Hemorragic fever |
| Mode of Transmission | Zoonosis, contact with body fluids |
| VIPR DB link | http://www.viprbrc.org/brc/vipr_allSpecies_search.do?method=SubmitForm&decorator=filo |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/197/filoviridae |
| Virus Host DB link | N.A. |
| Paper Title | Characterization of human and murine T-Cell immunoglobulin mucin domain 4 (TIM-4) IgV domain residues critical for Ebola virus entry |
| Author's Name | Bethany A. Rhein, Rachel B. Brouillette, Grace A. Schaack, John A. Chiorini, Wendy Maury |
| Journal Name | Journal Of Virology |
| Pubmed ID | 27122575 |
| Abstract | Phosphatidylserine (PtdSer) receptors that are responsible for the clearance of dying cells have recently been found to mediate enveloped virus entry. Ebola virus (EBOV), a member of the Filoviridae family of viruses, utilizes PtdSer receptors for entry into target cells. The PtdSer receptors human and murine T-cell immunoglobulin mucin (TIM) domain proteins TIM-1 and TIM-4 mediate filovirus entry by binding to PtdSer on the virion surface via a conserved PtdSer binding pocket within the amino-terminal IgV domain. While the residues within the TIM-1 IgV domain that are important for EBOV entry are characterized, the molecular details of virion-TIM-4 interactions have yet to be investigated. As sequences and structural alignments of the TIM proteins suggest distinct differences in the TIM-1 and TIM-4 IgV domain structures, we sought to characterize TIM-4 IgV domain residues required for EBOV entry. Using vesicular stomatitis virus pseudovirions bearing EBOV glycoprotein (EBOV GP/VSVΔG), we evaluated virus binding and entry into cells expressing TIM-4 molecules mutated within the IgV domain, allowing us to identify residues important for entry. Similar to TIM-1, residues in the PtdSer binding pocket of murine and human TIM-4 (mTIM-4 and hTIM-4) were found to be important for EBOV entry. However, additional TIM-4-specific residues were also found to impact EBOV entry, with a total of 8 mTIM-4 and 14 hTIM-4 IgV domain residues being critical for virion binding and internalization. Together, these findings provide a greater understanding of the interaction of TIM-4 with EBOV virions. |
| Used Model | HEK 293T and HEK 293 cells |
| DOI | 10.1128/JVI.00100-16 |
