| Gene Name | CTTN |
| HF Protein Name | Cortactin |
| HF Function | Inhibit Virus infection |
| Uniprot ID | Q14247 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 2017 |
| Host Factor (HF) Name in Paper | CTTN |
| Gene synonyms | EMS1 |
| Ensemble Gene ID | ENSG00000085733 |
| Ensemble Transcript | ENST00000301843.13 [Q14247-1];ENST00000346329.7 [Q14247-3];ENST00000376561.7 [Q14247-2];ENST00000671805.1 [Q14247-1];ENST00000671849.1 [Q14247-2];ENST00000672198.1 [Q14247-3] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0001726, GO:0002102, GO:0005522, GO:0005737, GO:0005783, GO:0005794, GO:0005829, GO:0005856, GO:0005886, GO:0005905, GO:0005925, GO:0005938, GO:0006886, GO:0006898, GO:0006930, GO:0007165, GO:0008076, GO:0030027, GO:0030036, GO:0030139, GO:0030516, GO:0030838, GO:0030863, GO:0043197, GO:0043231, GO:0045296, GO:0045987, GO:0048041, GO:0048812, GO:0048870, GO:0097062, GO:0097191, GO:0097581, GO:1901524, GO:1990023, GO:2001237, |
| MINT ID | Q14247 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 164765 |
| PANTHER ID | PTHR10829;PTHR10829:SF15 |
| PDB ID(s) | 1X69, 2D1X, |
| pfam ID | PF02218, PF14604, |
| Drug Bank ID | N.A., |
| ChEMBL ID | CHEMBL4295820 |
| Organism | Homo sapiens (Human) |
| Virus Name | Nipah Virus |
| Virus Short Name | NiV |
| Order | Mononegavirales |
| Virus Family | Paramyxoviridae |
| Virus Subfamily | N.A. |
| Genus | Henipavirus |
| Species | Nipah henipavirus |
| Host | Vertebrates |
| Cell Tropism | N.A. |
| Associated Disease | Fever and headache |
| Mode of Transmission | Sexual contact, blood, breast feeding |
| VIPR DB link | https://www.viprbrc.org/brc/vipr_search.do?species=Nipah_virus |
| ICTV DB link | https://ictv.global/report/152/paramyxoviridae |
| Virus Host DB link |
| Paper Title | Interaction of Nipah Virus F and G with the Cellular Protein Cortactin Discovered by a Proximity Interactome Assay |
| Author's Name | Chunmei Cui, Pengfei Hao, Chaozhi Jin, Wang Xu, Yuchen Liu, Letian Li, Shouwen Du, Limin Shang, Xin Jin, Ningyi Jin, Jian Wang, Chang Li |
| Journal Name | International Journal of Molecular Sciences |
| Pubmed ID | 38612921 |
| Abstract | Nipah virus (NiV) is a highly lethal zoonotic virus with a potential large-scale outbreak, which poses a great threat to world health and security. In order to explore more potential factors associated with NiV, a proximity labeling method was applied to investigate the F, G, and host protein interactions systematically. We screened 1996 and 1524 high-confidence host proteins that interacted with the NiV fusion (F) glycoprotein and attachment (G) glycoprotein in HEK293T cells by proximity labeling technology, and 863 of them interacted with both F and G. The results of GO and KEGG enrichment analysis showed that most of these host proteins were involved in cellular processes, molecular binding, endocytosis, tight junction, and other functions. Cytoscape software (v3.9.1) was used for visual analysis, and the results showed that Cortactin (CTTN), Serpine mRNA binding protein 1 (SERBP1), and stathmin 1 (STMN1) were the top 20 proteins and interacted with F and G, and were selected for further validation. We observed colocalization of F-CTTN, F-SERBP1, F-STMN1, G-CTTN, G-SERBP1, and G-STMN1 using confocal fluorescence microscopy, and the results showed that CTTN, SERBP1, and STMN1 overlapped with NiV F and NiV G in HEK293T cells. Further studies found that CTTN can significantly inhibit the infection of the Nipah pseudovirus (NiVpv) into host cells, while SERBP1 and STMN1 had no significant effect on pseudovirus infection. In addition, CTTN can also inhibit the infection of the Hendra pseudovirus (HeVpv) in 293T cells. In summary, this study revealed that the potential host proteins interacted with NiV F and G and demonstrated that CTTN could inhibit NiVpv and HeVpv infection, providing new evidence and targets for the study of drugs against these diseases. |
| Used Model | HEK293T |
| DOI | 10.3390/ijms25074112 |
