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Virus Details


VHFID9952

Host Factor Information

Pathogen Information

Publication Information

Host Factor Information

Gene Name ALDOA
HF Protein Name Fructose-bisphosphate aldolase A
HF Function
Uniprot ID P04075
Protein Sequence View Fasta Sequence
NCBI Gene ID 226
Host Factor (HF) Name in Paper ALDOA
Gene synonyms ALDA
Ensemble Gene ID ENSG00000149925
Ensemble Transcript ENST00000412304.6 [P04075-1];ENST00000563060.6 [P04075-1];ENST00000569545.5 [P04075-1];ENST00000642816.3 [P04075-2];ENST00000643777.4 [P04075-1]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0003723, GO:0003779, GO:0004332, GO:0005576, GO:0005615, GO:0005634, GO:0005829, GO:0006000, GO:0006096, GO:0006754, GO:0006941, GO:0007015, GO:0007339, GO:0008092, GO:0008360, GO:0015629, GO:0015631, GO:0016020, GO:0030388, GO:0031093, GO:0031430, GO:0031674, GO:0034774, GO:0042802, GO:0045296, GO:0046716, GO:0051289, GO:0061827, GO:0070061, GO:0070062, GO:1904724, GO:1904813,
MINT ID P04075
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 103850;611881
PANTHER ID PTHR11627;PTHR11627:SF1
PDB ID(s) 1ALD, 2ALD, 4ALD, 5KY6, 6XMH, 6XML, 6XMM, 6XMO,
pfam ID PF00274,
Drug Bank ID DB04733, DB02512, DB11638, DB04326, DB08240, DB01593, DB14487, DB14533, DB14548,
ChEMBL ID CHEMBL210
Organism Homo sapiens (Human)

Pathogen Information

Virus Name Japanese encephalitis virus
Virus Short Name JEV
Order Amarillovirales
Virus Family Flaviviridae
Virus Subfamily N.A.
Genus Flavivirus
Species Japanese encephalitis virus
Host Vertebrates
Cell Tropism Neurons
Associated Disease Encephalitis
Mode of Transmission Sexual contact, blood, breast feeding
VIPR DB link https://www.viprbrc.org/brc/vipr_search.do?species=Japanese_encephalitis_virus
ICTV DB link https://ictv.global/report/183/flaviviridae
Virus Host DB link

Publication Information

Paper Title Analysis of the Zika and Japanese Encephalitis Virus NS5 Interactomes
Author's Name Duangnapa Kovanich, Chonticha Saisawang, Potchaman Sittipaisankul, Suwipa Ramphan, Nuttiya Kalpongnukul, Poorichaya Somparn, Trairak Pisitkun, Duncan R Smith
Journal Name Journal of Proteome Research
Pubmed ID 31199156
Abstract Mosquito-borne flaviviruses, including dengue virus (DENV), Japanese encephalitis virus (JEV), and Zika virus (ZIKV), are major human pathogens. Among the flaviviral proteins, the nonstructural protein 5 (NS5) is the largest, most conserved, and major enzymatic component of the viral replication complex. Disruption of the common key NS5-host protein-protein interactions critical for viral replication could aid in the development of broad-spectrum antiflaviviral therapeutics. Hundreds of NS5 interactors have been identified, but these are mostly DENV-NS5 interactors. To this end, we sought to investigate the JEV- and ZIKV-NS5 interactomes using EGFP immunoprecipitation with label-free quantitative mass spectrometry analysis. We report here a total of 137 NS5 interactors with a significant enrichment of spliceosomal and spliceosomal-associated proteins. The transcription complex Paf1C and phosphatase 6 were identified as common NS5-associated complexes. PAF1 was shown to play opposite roles in JEV and ZIKV infections. Additionally, we validated several NS5 targets and proposed their possible roles in infection. These include lipid-shuttling proteins OSBPL9 and OSBPL11, component of RNAP3 transcription factor TFIIIC, minichromosome maintenance, and cochaperone PAQosome. Mining this data set, our study expands the current interaction landscape of NS5 and uncovers several NS5 targets that are new to flavivirus biology.
Used Model HEK293T/17
DOI 10.1021/acs.jproteome.9b00339