| Gene Name | PAF1 |
| HF Protein Name | RNA polymerase II-associated factor 1 homolog |
| HF Function | |
| Uniprot ID | Q8N7H5 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 54623 |
| Host Factor (HF) Name in Paper | PAF1 |
| Gene synonyms | PD2 |
| Ensemble Gene ID | ENSG00000006712 |
| Ensemble Transcript | ENST00000221265.8 [Q8N7H5-1];ENST00000595564.5 [Q8N7H5-3] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000122, GO:0000993, GO:0001650, GO:0001711, GO:0003682, GO:0005654, GO:0005737, GO:0006368, GO:0016020, GO:0016055, GO:0016593, GO:0019827, GO:0031124, GO:0034504, GO:0043231, GO:0045638, GO:0045944, GO:0071222, GO:1902808, |
| MINT ID | Q8N7H5 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 610506 |
| PANTHER ID | PTHR23188;PTHR23188:SF12 |
| PDB ID(s) | 4M6T, 5ZYQ, 6GMH, 6TED, 7OOP, 7OPC, 7OPD, 7UNC, 7UND, |
| pfam ID | PF03985, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Japanese encephalitis virus |
| Virus Short Name | JEV |
| Order | Amarillovirales |
| Virus Family | Flaviviridae |
| Virus Subfamily | N.A. |
| Genus | Flavivirus |
| Species | Japanese encephalitis virus |
| Host | Vertebrates |
| Cell Tropism | Neurons |
| Associated Disease | Encephalitis |
| Mode of Transmission | Sexual contact, blood, breast feeding |
| VIPR DB link | https://www.viprbrc.org/brc/vipr_search.do?species=Japanese_encephalitis_virus |
| ICTV DB link | https://ictv.global/report/183/flaviviridae |
| Virus Host DB link |
| Paper Title | Analysis of the Zika and Japanese Encephalitis Virus NS5 Interactomes |
| Author's Name | Duangnapa Kovanich, Chonticha Saisawang, Potchaman Sittipaisankul, Suwipa Ramphan, Nuttiya Kalpongnukul, Poorichaya Somparn, Trairak Pisitkun, Duncan R Smith |
| Journal Name | Journal of Proteome Research |
| Pubmed ID | 31199156 |
| Abstract | Mosquito-borne flaviviruses, including dengue virus (DENV), Japanese encephalitis virus (JEV), and Zika virus (ZIKV), are major human pathogens. Among the flaviviral proteins, the nonstructural protein 5 (NS5) is the largest, most conserved, and major enzymatic component of the viral replication complex. Disruption of the common key NS5-host protein-protein interactions critical for viral replication could aid in the development of broad-spectrum antiflaviviral therapeutics. Hundreds of NS5 interactors have been identified, but these are mostly DENV-NS5 interactors. To this end, we sought to investigate the JEV- and ZIKV-NS5 interactomes using EGFP immunoprecipitation with label-free quantitative mass spectrometry analysis. We report here a total of 137 NS5 interactors with a significant enrichment of spliceosomal and spliceosomal-associated proteins. The transcription complex Paf1C and phosphatase 6 were identified as common NS5-associated complexes. PAF1 was shown to play opposite roles in JEV and ZIKV infections. Additionally, we validated several NS5 targets and proposed their possible roles in infection. These include lipid-shuttling proteins OSBPL9 and OSBPL11, component of RNAP3 transcription factor TFIIIC, minichromosome maintenance, and cochaperone PAQosome. Mining this data set, our study expands the current interaction landscape of NS5 and uncovers several NS5 targets that are new to flavivirus biology. |
| Used Model | HEK293T/17 |
| DOI | 10.1021/acs.jproteome.9b00383 |
